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Enzyme Assays

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Author by : Robert Eisenthal
Languange Used : en
Release Date : 2002
Publisher by : Practical Approach

ISBN : 0199638209

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences....

Enzyme Assays

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Author by : Jean-Louis Reymond
Languange Used : en
Release Date : 2006-05-12
Publisher by : John Wiley & Sons

ISBN : 9783527607211

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists....

Practical Enzymology

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Author by : Hans Bisswanger
Languange Used : en
Release Date : 2019-10-21
Publisher by : John Wiley & Sons

ISBN : 9783527346042

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination....

Automated Enzyme Assays

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Author by : D.B. Roodyn
Languange Used : en
Release Date : 1970-01-01
Publisher by : Elsevier

ISBN : 9780080887739

In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends. It provides information on: principles and terminology of enzyme automation; automatic methods illustrated by the Technicon system; semi-automatic methods; interrupted-flow and discrete-sampling systems; single-enzyme analysis; multiple-enzyme analysis (M.E.A.); enzyme characterization; calculation of enzyme activities from instrument readings; generalized systems for enzyme automation; Appendices I. Published automated enzyme assays, II. Terminology used in enzyme automation, III. Apparatus used in enzyme automation, IV. Computer program for generalized enzyme automated system....

Enzyme Activity In Single Cells

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Author by :
Languange Used : en
Release Date : 2019-10-31
Publisher by : Academic Press

ISBN : 9780128170915

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Enzyme Activity in Single Cells...

Laboratory Guide To Enzymology

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Author by : Geoffrey A. Holdgate
Languange Used : en
Release Date : 2024-04-09
Publisher by : John Wiley & Sons

ISBN : 9781394179794

Understand how drug discovery incorporates the building blocks of complex life with this accessible guide Enzymes are among the fundamental building blocks of life, the proteins that catalyze some of the most important metabolic and biochemical reactions that sustain the complex organism. Understanding the unique structures and mechanisms of individual enzymes is key to medical treatment and pharmaceutical intervention, making it an especially essential aspect of drug discovery. The topic can appear challenging, however, as it requires command of interdisciplinary research combining biology, chemistry, and mathematics. Laboratory Guide to Enzymology brings these disciplines together to offer a practical, accessible overview of the lab-based work of creating effective enzyme essays. Beginning with a guide to the fundamentals of enzyme theory, the book connects that theory to a range of modern methods of computation and experimentation. The result is a detailed but highly readable volume elucidating the cornerstone of drug discovery research. Laboratory Guide to Enzymology readers will also find: Specific examples of enzyme application in drug discovery Detailed discussion of topics including enzyme catalysis, chemical kinetics, steady-state assays, and many more Appendices including a range of vital supplementary material Laboratory Guide to Enzymology is ideal for all biomedical and pharmaceutical researchers working in enzymology and assay development, as well as advanced students in the biomedical or biochemical sciences looking to develop a working knowledge of this field of research....

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Enzyme Assays

Enzyme Assays

Practical Enzymology

Automated Enzyme Assays

Enzyme Activity In Single Cells

Laboratory Guide To Enzymology

Enzyme Assays

Enzyme Assays

Enzyme Assays For Food Scientists

Enzyme Assays For Food Scientists