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Practical Enzymology

Practical Enzymology

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Author by : Hans Bisswanger
Languange Used : en
Release Date : 2013-06-21
Publisher by : John Wiley & Sons

ISBN : 9783527659241

The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter....



A Practical Guide To Enzymology

A Practical Guide To Enzymology

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Author by : Clarence H. Suelter
Languange Used : en
Release Date : 1985-11-22
Publisher by : Wiley-Interscience

ISBN : UCAL:B4274581

Provided in this book are guidelines and practical advice for anyone working with enzymes....



Practical Clinical Enzymology

Practical Clinical Enzymology

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Author by : John King
Languange Used : en
Release Date : 1965
Publisher by :

ISBN : WISC:89043235514

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Enzymes

Enzymes

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Author by : Robert A. Copeland
Languange Used : en
Release Date : 2004-04-07
Publisher by : John Wiley & Sons

ISBN : 9780471461852

Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research....



Practical Enzymology

Practical Enzymology

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Author by : Hans Bisswanger
Languange Used : en
Release Date : 2019-10-21
Publisher by : John Wiley & Sons

ISBN : 9783527346042

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination....



Practical Enzymology

Practical Enzymology

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Author by : Austin Murphy
Languange Used : en
Release Date : 2022-09-20
Publisher by :

ISBN : 1639874453

Biological catalysts which assist in accelerating certain chemical reactions are called enzymes. These proteins are highly specific in nature and can only act under the optimal temperature and pressure. On exposure to excessive heat, enzymes get denatured and lose their catalytic properties. Enzymology is defined as the study of the kinetics, structures and function of these enzymes. The important biological applications of enzymes involve metabolism, transcription, muscle contraction, hormone release, etc. They also find use in the dairy industry, paper industry, brewing industry, biofuel industry and food processing. On the basis of functions performed, enzymes can be classified into oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. This book elucidates the concepts and innovative models around prospective developments with respect to enzymology. The various sub-fields of enzymology along with technological progress that have future implications are glanced at herein. Coherent flow of topics, student-friendly language and extensive use of examples make this book an invaluable source of knowledge....



Practical Enzymology Of The Sphingolipidoses

Practical Enzymology Of The Sphingolipidoses

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Author by : Robert H. Glew
Languange Used : en
Release Date : 1977
Publisher by :

ISBN : UOM:39015000890130

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Enzymes

Enzymes

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Author by : Robert Allen Copeland
Languange Used : en
Release Date : 1996
Publisher by : Wiley-VCH

ISBN : UOM:39015037847798

Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research....



Enzyme Assays

Enzyme Assays

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Author by : Robert Eisenthal
Languange Used : en
Release Date : 1992
Publisher by : Oxford University Press, USA

ISBN : UOM:39015036161241

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences....



Practical Enzymology Of The Sphingolipidoses Lrmbm

Practical Enzymology Of The Sphingolipidoses Lrmbm

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Author by : Glew
Languange Used : en
Release Date : 1977-05
Publisher by :

ISBN : 0471564060

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